| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | SCHWANKE, Mary | SR | North Shelby High School | 22.13 | Verified |
| 4 | RUBA, Sadie | SR | Green City High School | 21.84 | Verified |
| 5 | HOLLENDER, Brinley | JR | Clark County High School | 22.09 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBERTS, Hailey | SO | Schuyler County High School | 20.04 | Verified |
| 2 | CRUSHA, Sophia | FR | Paris High School | 18.00 | Unverified: |
| 3 | FOWLER, Madison | JR | Schuyler County High School | 17.54 | Verified |
| 4 | HERBST, Jersey | JR | Kirksville High School | 16.61 | Verified |
| 5 | LYONS, Dawsyn | SR | Kirksville High School | 16.64 | Verified |
| 6 | FREDERICK, Kelsey | SO | Novinger High School | 17.92 | Verified |
| 7 | CHRISTENSEN, Sylvie | JR | Atlanta High School | 18.92 | Verified |