| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCMANN, Marissa | FR | Brashear High School | NT | Unverified: |
| 2 | HEIMER, Meradith | FR | Kirksville High School | NT | Unverified: |
| 3 | LANHAM, Allie | JR | Schuyler County High School | NT | Unverified: |
| 4 | KIRBY, Ceairra | SR | North Shelby High School | NT | Unverified: |
| 5 | GORDON, Haylee | SO | Schuyler County High School | NT | Unverified: |
| 6 | MELNICK, Tyne | SO | Kirksville High School | NT | Unverified: |
| 7 | HERBST, Jersey | JR | Kirksville High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CISNEROS, Jazmin | FR | Milan High School | NT | Unverified: |
| 2 | WEST, Kamy | JR | Atlanta High School | 38.20 | Verified |
| 3 | RUMMERFIELD, Zailey | SO | Kirksville High School | 34.93 | Verified |
| 4 | CLARK, Paislee | FR | Kirksville High School | 32.01 | Verified |
| 5 | MOOTS, MADDIE | FR | Brashear High School | 33.74 | Verified |
| 6 | HOLLENDER, Brinley | JR | Clark County High School | 35.06 | Verified |
| 7 | SNYDER, Allyson | FR | Kirksville High School | NT | Unverified: |
| 8 | MCKAY, AVA | SO | Clark County High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | VICE, Faith | FR | Kirksville High School | 31.58 | Verified |
| 2 | STARKEY, Catherine | SO | Kirksville High School | 30.97 | Verified |
| 3 | AESCHILMAN, Sidney | JR | Schuyler County High School | 29.64 | Verified |
| 4 | SMITH, Annamarie | SO | Milan High School | 29.33 | Verified |
| 5 | SHOEMYER, Rachel | SR | Paris High School | 29.40 | Unverified: |
| 7 | BORGMEYER, Alexa | JR | Brashear High School | 31.13 | Verified |
| 8 | HENDREN, Khloee | FR | Paris High School | 32.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARRIS, Mya | FR | Kirksville High School | 28.90 | Verified |
| 2 | TETER, Makaila | SR | Atlanta High School | 28.38 | Verified |
| 3 | PICKERING, Amy | SO | Milan High School | 27.26 | Verified |
| 4 | SHINN, Whitney | SR | North Shelby High School | 26.72 | Verified |
| 5 | MORRIS, Jacie | SR | Schuyler County High School | 26.94 | Verified |
| 7 | COPENHAVER, Harper | FR | North Shelby High School | 28.65 | Unverified: |
| 8 | BAUMGARTNER, Adison | JR | Kirksville High School | 29.10 | Verified |