| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Drew | SR | Paris High School | 2:10.00 | Unverified: |
| 2 | SCHUTTER, Justin | FR | Kirksville High School | 2:19.03 | Verified |
| 3 | DAY, Austin | SO | Clark County High School | 2:22.46 | Verified |
| 4 | PHILLIPS, Roman | SR | Atlanta High School | 2:23.73 | Verified |
| 5 | GRISSOM, CALEB | FR | Kirksville High School | 2:31.80 | Verified |
| 6 | LITTRELL, Ivan | FR | Green City High School | 2:32.44 | Verified |
| 7 | NELSON, Cooper | FR | Atlanta High School | 2:34.03 | Verified |
| 8 | NOE, Clancy | JR | Brashear High School | 2:38.01 | Verified |
| 9 | MCATEE, Koan | JR | Kirksville High School | 2:41.53 | Verified |
| 10 | BURCH, Trevor | FR | Paris High School | 2:49.00 | Unverified: |
| 11 | HARRISON, Tristin | SO | Green City High School | 2:54.00 | Verified |
| 12 | SNYDER, Jacob | SO | Brashear High School | 2:54.64 | Verified |
| 13 | CRIST, Jake | FR | Green City High School | NT | Unverified: |
| 14 | WHITLOW, Riley | SO | Brashear High School | NT | Unverified: |
| 15 | THOMPSON, Brayden | SO | Clark County High School | NT | Unverified: |
| 17 | KLYUKOVSKI, Gregory | FR | Kirksville High School | NT | Unverified: |
| 18 | NOVENCIDO, Andrew | SR | Kirksville High School | NT | Unverified: |
| 19 | GRIEGO, Merritt | SO | Kirksville High School | NT | Unverified: |
| 20 | YOS, Luke | FR | Novinger High School | NT | Unverified: |
| 21 | DUNN, Floyd | FR | Kirksville High School | NT | Unverified: |
| 22 | WARDLOW, Isaac | SO | Clark County High School | NT | Unverified: |