| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TALLMAN, Kelsey | JR | Schuyler County High School | 2:43.80 | Verified |
| 2 | MAREK, Kiersten | SR | Kirksville High School | 2:47.59 | Verified |
| 3 | CARTER, Avery | FR | Novinger High School | 2:51.74 | Verified |
| 4 | CULLERS, Xavia | SR | Schuyler County High School | 2:54.29 | Verified |
| 5 | GORDON, Haylee | SO | Schuyler County High School | 2:57.41 | Verified |
| 6 | UHLMEYER, Addy | SO | North Shelby High School | 2:59.54 | Verified |
| 7 | EMMA, Ashenfelter | FR | Paris High School | 3:00.00 | Unverified: |
| 8 | HODGES, Makenna | SO | Clark County High School | 3:06.91 | Verified |
| 9 | SIZEMORE, KAYLEE | FR | Brashear High School | 3:14.53 | Verified |
| 10 | CARTER, Elizabeth | FR | Novinger High School | 3:19.74 | Verified |
| 11 | ORTEGA, Milly | FR | Green City High School | 3:28.99 | Verified |
| 12 | ORTEGA, Mia | SO | Green City High School | NT | Unverified: |
| 13 | STONEBURNER, Lilly | SO | North Shelby High School | NT | Unverified: |
| 15 | WILEY, Adrien | FR | North Shelby High School | NT | Unverified: |
| 16 | BOONE, Patience | FR | Atlanta High School | NT | Unverified: |