| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SCHWANKE, Mary | SR | North Shelby High School | 58.95 | Verified |
| 3 | FREDERICK, Kelsey | SO | Novinger High School | 54.61 | Verified |
| 4 | HERBST, Jersey | JR | Kirksville High School | 46.64 | Verified |
| 5 | CHRISTENSEN, Sylvie | JR | Atlanta High School | 53.98 | Verified |
| 6 | CRUSHA, Sophia | FR | Paris High School | 58.00 | Unverified: |
| 7 | HOLLENDER, Brinley | JR | Clark County High School | 1:01.98 | Verified |