| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | CRABTREE, Rylynn | SO | Schuyler County High School | NT | Unverified: |
| 4 | HODGES, Makenna | SO | Clark County High School | 1:16.28 | Verified |
| 5 | FARRIS, Hannah | FR | Schuyler County High School | NT | Unverified: |
| 6 | BOONE, Patience | FR | Atlanta High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HENDREN, Khloee | FR | Paris High School | 1:12.00 | Unverified: |
| 2 | MAREK, Kiersten | SR | Kirksville High School | 1:08.64 | Verified |
| 3 | LINBERGER, Caroline | SR | North Shelby High School | 1:04.96 | Verified |
| 4 | SHINN, Whitney | SR | North Shelby High School | 59.93 | Verified |
| 5 | THOMAS, Maya | SR | Kirksville High School | 1:04.46 | Verified |
| 6 | BORGMEYER, Alexa | JR | Brashear High School | 1:07.21 | Verified |
| 7 | LANHAM, Allie | JR | Schuyler County High School | 1:08.76 | Verified |