| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SCHUTTER, Justin | FR | Kirksville High School | 4:52.69 | Verified |
| 2 | KECK, Nathan | SR | Milan High School | 5:06.21 | Verified |
| 3 | DEORNELLAS, Brayden | SR | Paris High School | 5:20.00 | Unverified: |
| 4 | CHRISTENSEN, Carter | SO | Atlanta High School | 5:22.56 | Verified |
| 5 | VALLADARES, Christopher | SO | Milan High School | 5:23.49 | Verified |
| 6 | CHAPMAN, Landen | FR | Paris High School | 5:25.00 | Unverified: |
| 7 | KIMBRELL, Gabriel | SO | Schuyler County High School | 5:30.31 | Verified |
| 8 | GRISSOM, CALEB | FR | Kirksville High School | 5:35.49 | Verified |
| 9 | SNYDER, Jacob | SO | Brashear High School | 5:59.65 | Verified |
| 11 | YOS, Luke | FR | Novinger High School | 6:31.41 | Verified |
| 12 | WHITLOW, Riley | SO | Brashear High School | NT | Unverified: |
| 13 | THOMPSON, Brayden | SO | Clark County High School | NT | Unverified: |
| 14 | APPLEGATE, Asher | FR | Schuyler County High School | NT | Unverified: |
| 15 | HATCHER, Keaton | JR | Atlanta High School | NT | Unverified: |
| 16 | HURT, Kindric | SO | Clark County High School | NT | Unverified: |
| 17 | AUBUCHON, Eric | FR | Kirksville High School | NT | Unverified: |