| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Mehlville High School | A | Nasufovic | O'Shea | Hastings | TREVISANO | 1:58.72 | Verified |
| 4 | Webster Groves High School | A | Dill | Logan | Lawless | Rucker | 1:53.14 | Verified |
| 5 | Rockwood Summit High School | A | 1:53.53 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | A | 1:53.00 | Verified | ||||
| 2 | MICDS | A | Brothers | O'Basuyi | Goodwin | Niemeyer | 1:51.52 | Verified |
| 3 | Parkway West High School | A | Love | Fotheringham | Watts | Grunzinger | 1:49.68 | Verified |
| 4 | Hazelwood Central High School | A | Wilkins | Mckenzie | Stamps | Reed | 1:48.47 | Verified |
| 5 | Eureka High School | A | 1:49.03 | Verified | ||||
| 6 | Marquette High School | A | DAVIS | Haymon | NEAL | QIN | 1:51.16 | Verified |
| 7 | Parkway Central High School | A | NT | Verified | ||||
| 8 | Oakville High School | A | Czuppon | JONAS | SQUIRES | Mueller | 1:53.11 | Verified |