| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Parkway Central High School | A | Benoist | Lee | Fritz | Green | 2:03.00 | Unverified: |
| 2 | Lafayette (Wildwood) High School | A | 2:00.86 | Unverified: | ||||
| 3 | Eureka High School | A | 1:56.03 | Unverified: | ||||
| 4 | Mehlville High School | A | Shores | Hastings | TREVISANO | Ramos | 2.00 | Unverified: Oakville Inv |
| 5 | Rockwood Summit High School | A | 1:55.00 | Unverified: | ||||
| 6 | Hazelwood Central High School | A | Butler | Wilkins | Reed | Elam | 1:56.24 | Unverified: |
| 7 | Webster Groves High School | A | Dill | Harrison | Juelich | Trevino | 2:02.00 | Unverified: |