| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | MICDS | A | Phillips | Crawford | Niemeyer | Wells | 5:21.96 | Verified |
| 2 | Rockwood Summit High School | B | 2:20.25 | Unverified: | ||||
| 3 | Webster Groves High School | B | Blum | Nguyen | Cannon | Miller | 2:20.00 | Unverified: |
| 4 | Mehlville High School | A | Wheeler | Burnett | Alhalabi | O'Brien | 2:14.00 | Unverified: Oakville Inv |
| 5 | Parkway West High School | A | Josef | Phillips | Sanders | Masalskis | 2:15.00 | Unverified: |
| 6 | Lafayette (Wildwood) High School | B | 2:20.00 | Unverified: | ||||
| 7 | Mehlville High School | B | Nava | Wilder | Brindley | Mask | 2:25.20 | Unverified: Oakville Inv |
| 8 | Oakville High School | A | DRESCH | Lyons | Pleimann | THARP DICKERSON | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Seckman High School | B | Mattler | Wynn | Dundon | Summers | 2:12.00 | Unverified: |
| 2 | Hazelwood Central High School | A | Miles | Chairse | White | Jones | 2:08.24 | Unverified: |
| 3 | Eureka High School | B | 2:04.50 | Unverified: | ||||
| 4 | Seckman High School | A | Morie | Zilch | Crowley | Easter | 2:02.00 | Unverified: |
| 5 | Eureka High School | A | 2:03.22 | Unverified: | ||||
| 6 | Rockwood Summit High School | A | 2:05.25 | Unverified: | ||||
| 7 | Lafayette (Wildwood) High School | A | 2:10.00 | Unverified: | ||||
| 8 | Webster Groves High School | A | Knox | Robinson | Fischer | McCoy | 2:13.00 | Unverified: |