| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Mehlville High School | A | Barrett | Abdijanovic | Burch | Dizdarevic | 4:46.09 | Verified |
| 4 | Parkway West High School | A | Drake | Fotheringham | Watts | Grunzinger | 4:27.40 | Verified |
| 5 | Parkway Central High School | A | Fritz | Piepho | Benoist | Williams | 4:29.84 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Oakville High School | A | Czuppon | WESCOAT | BROWER | JONAS | 4:22.70 | Verified |
| 2 | MICDS | A | Goodwin | Coppel | O'Basuyi | Wyckoff | 4:21.19 | Verified |
| 3 | Rockwood Summit High School | A | 4:20.55 | Verified | ||||
| 4 | Lafayette (Wildwood) High School | A | 4:04.92 | Verified | ||||
| 5 | Eureka High School | A | 4:15.08 | Verified | ||||
| 6 | Webster Groves High School | A | Murdock | King | Allen | Logan | 4:20.58 | Verified |
| 7 | Hazelwood Central High School | A | Wilkins | Stamps | Catching | Elam | 4:21.53 | Verified |
| 8 | Marquette High School | A | QIN | CLARK | EHIMARE | Schweitzer | 4:24.29 | Verified |