| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Mehlville High School | B | Mask | Dang | Mask | Brindley | NT | Unverified: |
| 4 | Mehlville High School | A | O'Brien | McClusky | Gutjahr | Roberts | 5:03.45 | Unverified: |
| 5 | Sullivan High School | A | Evans | Chambers | Cabanlit | Prater | 5:27.93 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marquette High School | B | Bolt | BOLT | Bhatia | Wrisberg | 4:55.00 | Unverified: |
| 2 | Eureka High School | B | 4:50.00 | Unverified: | ||||
| 3 | Rockwood Summit High School | B | 4:45.00 | Unverified: | ||||
| 4 | Marquette High School | A | Wilson | Terry | Rashman | Russell | 4:40.00 | Unverified: |
| 5 | Parkway Central High School | A | Mitchell | Anderson | Mosley | Burk | 4:45.00 | Unverified: |
| 6 | Parkway West High School | A | Masalskis | Josef | Sanders | Phillips | 4:50.00 | Unverified: |
| 7 | Webster Groves High School | A | Pierson | McCoy | Pierce | Perry | 4:50.00 | Unverified: |
| 8 | Lafayette (Wildwood) High School | B | 5:00.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood Central High School | B | Jones | Cannon | Greene | Harris | 4:36.53 | Unverified: |
| 2 | Hazelwood Central High School | A | White | Wicks | McCanery | Scruggs | 4:31.53 | Unverified: |
| 3 | Seckman High School | A | Dundon | Formby | Morie | Easter | NT | Unverified: |
| 4 | Oakville High School | A | Benignus | WALTON | Clark | Kadymova | 4:22.70 | Verified |
| 5 | Parkway West High School | B | Kucukkaya | Kamau | Jenkins | 4:27.40 | Verified | |
| 6 | Rockwood Summit High School | A | 4:30.00 | Verified | ||||
| 7 | Eureka High School | A | 4:35.00 | Unverified: | ||||
| 8 | Lafayette (Wildwood) High School | A | 4:40.00 | Unverified: |