| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | FRITZ, Zoe | SR | Parkway Central High School | 27.97 |
| 2 | STAMPS, Ryann | FR | Hazelwood Central High School | 27.59 |
| 3 | MCKENZIE, Mya | SR | Hazelwood Central High School | 27.50 |
| 4 | BARAFUNDI, Christelle | JR | Rockwood Summit High School | 26.81 |
| 5 | JACKSON, Barrett | JR | Rockwood Summit High School | 26.97 |
| 6 | DAVIS, SAMARA | JR | Marquette High School | 27.56 |
| 7 | MITCHELL, Hannah | JR | Eureka High School | 27.88 |
| 8 | THIELE, Samantha | JR | Eureka High School | 28.02 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GARDNER, Layla | FR | Lafayette (Wildwood) High School | 32.00 | Unverified: |
| 2 | LEE, Karen | SR | Parkway Central High School | 28.84 | Verified |
| 3 | GRUNZINGER, Josie | SO | Parkway West High School | 28.21 | Verified |
| 4 | BARAFUNDI, Christelle | JR | Rockwood Summit High School | 25.57 | Verified |
| 5 | BROTHERS, Skye | SR | MICDS | 28.20 | Verified |
| 6 | MUELLER, Jenna | SR | Oakville High School | 28.84 | Verified |
| 7 | TREVISANO, TAYLOR | SR | Mehlville High School | 31.22 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STEINNERD, Carlee | SR | Seckman High School | NT | Unverified: |
| 2 | DILL, Halle | FR | Webster Groves High School | 28.88 | Verified |
| 3 | HEADY, Eva | JR | Sullivan High School | 28.24 | Verified |
| 4 | JACKSON, Barrett | JR | Rockwood Summit High School | 25.79 | Verified |
| 5 | O'BASUYI, Edia | FR | MICDS | 28.16 | Verified |
| 6 | JONAS, MADYSEN | JR | Oakville High School | 28.84 | Verified |
| 7 | LOVE, Jamaya | FR | Parkway West High School | 30.62 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | LEITNER, Emily | SR | Seckman High School | 28.93 | Verified |
| 3 | FRITZ, Zoe | SR | Parkway Central High School | 28.28 | Verified |
| 4 | DAVIS, SAMARA | JR | Marquette High School | 26.64 | Verified |
| 5 | THIELE, Samantha | JR | Eureka High School | 28.02 | Verified |
| 6 | BENNETT, Akili | FR | Marquette High School | 28.58 | Verified |
| 7 | NASUFOVIC, Adna | JR | Mehlville High School | 29.77 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | RUCKER, Dasia | SO | Webster Groves High School | 29.15 | Verified |
| 3 | GOODRICH, JULIANA | JR | Lafayette (Wildwood) High School | 28.42 | Verified |
| 4 | MCKENZIE, Mya | SR | Hazelwood Central High School | 27.11 | Verified |
| 5 | MITCHELL, Hannah | JR | Eureka High School | 27.17 | Verified |
| 6 | STAMPS, Ryann | FR | Hazelwood Central High School | 28.44 | Verified |
| 7 | MCCLAIN, Daveah | SO | Sullivan High School | 29.49 | Verified |