| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TREVINO, Nylla | FR | Webster Groves High School | 56.47 | Verified |
| 2 | BANKS, Tristen | JR | Parkway West High School | 53.12 | Verified |
| 3 | CLARK, SARAH | JR | Marquette High School | 51.97 | Unverified: Henle Holmes Invite |
| 4 | ELAM, Marsae | JR | Hazelwood Central High School | 51.61 | Verified |
| 5 | WEISS, Makenna | JR | Lafayette (Wildwood) High School | 51.69 | Verified |
| 6 | ABDIJANOVIC, Anela | JR | Mehlville High School | 52.52 | Verified |
| 7 | BENOIST, Kate | JR | Parkway Central High School | 53.31 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | O'SHEA, Jenna | SR | Mehlville High School | 50.37 | Verified |
| 2 | WITHERSPOON, Stevie | SO | Hazelwood Central High School | 49.90 | Verified |
| 3 | WATTS, Cecelia | SR | Parkway West High School | 48.26 | Verified |
| 4 | ANDERSON, Maya | SR | MICDS | 42.74 | Verified |
| 5 | LEE, Skyye | JR | Parkway Central High School | 45.50 | Unverified: |
| 6 | MURDOCK, Samantha | JR | Webster Groves High School | 49.10 | Verified |
| 7 | GENTILINI, Olivia | SR | Eureka High School | 49.90 | Verified |
| 8 | KIRBY, Kennedi | JR | Eureka High School | 50.64 | Verified |