| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CAVAZOS, Emma | FR | Parkway Central High School | 1:06.07 | Verified |
| 3 | BOND, Yvonna | SR | Mehlville High School | 1:01.95 | Verified |
| 4 | HUGHES, PAIGE | SO | Oakville High School | 59.30 | Verified |
| 5 | CLARK, Jordyn | FR | Parkway Central High School | 1:01.35 | Verified |
| 6 | WHEELER, Julia | SO | Mehlville High School | 1:02.30 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KOMMU, Lasya | FR | Lafayette (Wildwood) High School | 58.53 | Verified |
| 2 | MU, Sarah | FR | Lafayette (Wildwood) High School | 57.53 | Verified |
| 3 | VANDERHOOF, Amara | FR | Eureka High School | 57.36 | Verified |
| 4 | HENROID, Lillian | JR | Parkway Central High School | 57.06 | Verified |
| 5 | HARRIS, Kayla | SO | Hazelwood Central High School | 57.16 | Verified |
| 6 | DOHERTY, GRACE | SO | Lafayette (Wildwood) High School | 57.48 | Verified |
| 7 | NOTTINGHAM, Ella | FR | Eureka High School | 58.43 | Verified |
| 8 | SUMMERS, Cora | FR | Seckman High School | 58.87 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TERRY, Demoria | FR | Marquette High School | 56.10 | Verified |
| 2 | ESKER, Lexi | SR | St. Joseph's Academy | 55.25 | Verified |
| 3 | FORMBY, Sabrina | FR | Seckman High School | 53.68 | Verified |
| 4 | REECE, Na'Kyia | JR | Hazelwood Central High School | 52.66 | Verified |
| 5 | CAMPBELL, Alyssa | FR | Rockwood Summit High School | 52.99 | Verified |
| 6 | ISLAM, SABRINA | JR | Oakville High School | 55.18 | Verified |
| 7 | CRINNION, Cameron | FR | Seckman High School | 55.40 | Verified |
| 8 | RASHMAN, Corinne | FR | Marquette High School | 56.81 | Verified |