| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | GREEN, Madelynn | SR | Mehlville High School | 1:15.00 | Unverified: Oakville Inv. |
| 3 | ASHOK, Riya | JR | Parkway West High School | 1:08.84 | Verified |
| 4 | WESCOAT, ELIZABETH | SO | Oakville High School | 1:08.03 | Verified |
| 5 | MORSE, Kathryn | SR | Eureka High School | 1:08.05 | Verified |
| 6 | IVANOVA, Betina | SR | Parkway West High School | 1:12.77 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ZIMMERMANN, Hailey | JR | Seckman High School | 1:07.87 | Verified |
| 2 | GOODWIN, Sophia | SO | MICDS | 1:06.71 | Verified |
| 3 | JONAS, MADYSEN | JR | Oakville High School | 1:06.24 | Verified |
| 4 | FRITZ, Zoe | SR | Parkway Central High School | 1:05.99 | Verified |
| 5 | LOGAN, Teuana | JR | Webster Groves High School | 1:06.18 | Verified |
| 6 | PALMER, Riley | SR | Mehlville High School | 1:06.25 | Verified |
| 7 | KING, Olivia | SR | Webster Groves High School | 1:07.00 | Verified |
| 8 | PIEPHO, Keri | SO | Parkway Central High School | 1:07.98 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SINGH, Shrutika | SR | Lafayette (Wildwood) High School | 1:05.00 | Verified |
| 2 | CATCHING, Aliya | JR | Hazelwood Central High School | 1:04.12 | Verified |
| 3 | WARD, Angela | SR | Seckman High School | 1:00.22 | Verified |
| 4 | BROWN, Avery | SO | Lafayette (Wildwood) High School | 58.00 | Verified |
| 5 | JACKSON, Barrett | JR | Rockwood Summit High School | 1:00.07 | Verified |
| 6 | SCHNURBUSCH, Raegan | JR | Eureka High School | 1:01.25 | Verified |
| 7 | POPLAWSKI, Lilly | JR | Rockwood Summit High School | 1:04.85 | Verified |
| 8 | BENNETT, Akili | FR | Marquette High School | 1:05.70 | Verified |