| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | THARP DICKERSON, ALAYNA | SO | Oakville High School | NT | Unverified: |
| 3 | JONES, Kimora | JR | Parkway West High School | NT | Unverified: |
| 4 | THOMPSON, Nylah | JR | Parkway West High School | NT | Unverified: |
| 5 | WALTON, MACEY | JR | Oakville High School | NT | Unverified: |
| 6 | MENGHINI, Sydney | FR | St. Joseph's Academy | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BOLT, Sarah | SR | Marquette High School | 1:26.66 | Verified |
| 2 | GUTJAHR, Madeline | FR | Mehlville High School | 1:23.00 | Unverified: |
| 3 | CLINE, Rachyl | FR | Seckman High School | 1:20.00 | Unverified: |
| 4 | PIERSON, Audrey | FR | Webster Groves High School | 1:17.43 | Verified |
| 5 | MILLER, Elisabeth | SO | Webster Groves High School | 1:19.12 | Verified |
| 6 | OTAJAGIC, Ajla | FR | Mehlville High School | 1:22.00 | Unverified: |
| 7 | EVANS, Peyton | JR | Sullivan High School | 1:23.24 | Verified |
| 8 | CHAMBERS, Gwendolynn | SR | Sullivan High School | 1:32.82 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAYES, Cherish | SO | Hazelwood Central High School | 1:15.53 | Verified |
| 2 | BROTHERTON, Anna | FR | Eureka High School | 1:14.00 | Unverified: |
| 3 | KAMINENI, Ananya | JR | MICDS | 1:13.58 | Verified |
| 4 | CANNON, Aaniyah | JR | Hazelwood Central High School | 1:13.03 | Verified |
| 5 | GIBSON, Wuaniata | FR | Marquette High School | 1:13.30 | Verified |
| 6 | KROBATH, LEAH | SO | Rockwood Summit High School | 1:13.63 | Verified |
| 7 | BESHIER, Sophia | FR | St. Joseph's Academy | NT | |
| 8 | CABANLIT, Zandra | SR | Sullivan High School | 1:15.75 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JONES, Trinity | JR | Hazelwood Central High School | 1:11.59 | Verified |
| 2 | GOOD, Ally | FR | St. Joseph's Academy | 1:11.00 | Verified |
| 3 | ROPER, Aubrey | FR | Rockwood Summit High School | 1:10.00 | Unverified: |
| 4 | WELLS, Natasha | FR | MICDS | 1:09.67 | Verified |
| 5 | WILSON, Kaitlin | FR | Marquette High School | 1:09.70 | Verified |
| 6 | MOSLEY, Kendall | FR | Parkway Central High School | 1:10.18 | Verified |
| 7 | TAYON, Hope | SO | Lafayette (Wildwood) High School | 1:11.02 | Verified |
| 8 | O'BRIEN, Natalie | SO | Mehlville High School | 1:12.86 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REED, Callista | SR | Eureka High School | 1:09.40 | Unverified: |
| 2 | LONG, Addison | FR | Lafayette (Wildwood) High School | 1:08.33 | Verified |
| 3 | BENIGNUS, Laura | FR | Oakville High School | 1:07.78 | Verified |
| 4 | STEVENS, Elliotte | FR | Rockwood Summit High School | 1:04.82 | Verified |
| 5 | MADISON, Rayne | SO | Parkway Central High School | 1:05.75 | Verified |
| 6 | MITCHELL, Ashlyn | FR | Parkway Central High School | 1:07.91 | Verified |
| 7 | CONTESTABILE, Kensington | SO | Eureka High School | 1:08.50 | Unverified: |
| 8 | DUNDON, Jamie | FR | Seckman High School | 1:09.52 | Verified |