| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Oakville High School | A | Mueller | SQUIRES | WESCOAT | WETTEROFF | 55.83 | Verified |
| 4 | Mehlville High School | A | Shores | Roberts | Palmer | O'Shea | 54.33 | Verified |
| 5 | Parkway Central High School | A | NT | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | A | Harrison | Juelich | Simon | Rucker | 53.61 | Verified |
| 2 | Lafayette (Wildwood) High School | A | 53.00 | Verified | ||||
| 3 | Rockwood Summit High School | A | 51.66 | Verified | ||||
| 4 | Hazelwood Central High School | A | Madison | Reed | Mckenzie | Wilkins | 49.61 | Verified |
| 5 | Eureka High School | A | 51.05 | Verified | ||||
| 6 | Seckman High School | A | Ward | Leitner | Sexton | Moeckel | 52.44 | Verified |
| 7 | MICDS | A | Brothers | O'Basuyi | Jiang | Niemeyer | 53.31 | Verified |
| 8 | Marquette High School | A | Cross | DAVIS | Haymon | QIN | 54.04 | Verified |