| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Kirkwood High School | A | Barry | Earl | Loper | Griffin | 47.00 | Unverified: |
| 3 | Chaminade College Preparatory Sc | A | Collins | Kai) Robinson | Dyson | Chris) Funches | 45.52 | Verified |
| 4 | Vianney High School | A | Ivory | Ruzicka | Pate | Pullam | 46.11 | Verified |
| 5 | St. Louis University High School | A | 52.13 | Unverified: |