| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Louis University High School | A | 1:46.20 | Unverified: | ||||
| 2 | Chaminade College Preparatory Sc | A | Butler | D.K.) Weaver | Dyson | Karcher | 1:41.28 | Verified |
| 3 | Vianney High School | A | Ivory | Ruzicka | Turmelle | Pullam | 1:38.03 | Verified |
| 4 | Kirkwood High School | A | Weinberg | Bergmann | Kring | Sammler | 1:40.00 | Unverified: |
| 5 | Northwest (Cedar Hill) High Scho | A | Frazier | Smith | Williams | Penberthy | 1:43.63 | Unverified: |