| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | JACKSON, MJ | JR | Tennessee-Martin | NT | |
| 4 | MARSHALL, Marques | SO | Tennessee-Martin | 25.05 | Margaret Simmons Invite 23 |
| 5 | COUPLAND, Sam | FR | Lindenwood | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SPELLS, Malakhai | SR | Lewis | 24.60 | |
| 2 | POWERS, Aubrey | FR | Wis.-Parkside | 24.22 | Parkside Classic |
| 3 | FOSTER, Dorian | FR | McKendree | 24.15 | Miner Invitational |
| 4 | PRESSLEY, Isaiah | SO | Southern Illinois | 23.91 | 2023 Notre Dame Invitational |
| 5 | SALAS, Biel | FR | Lindenwood | 23.97 | 2023 EIU Big Blue |
| 6 | PINNICK, Aaron | FR | Lewis | 24.18 | Miner Invitational |
| 7 | PEARL, Caden | FR | Lewis | 24.23 | Lewis Invitational |
| 8 | BONDS, Cam'ron | FR | Tennessee-Martin | 24.69 | Margaret Simmons Invite 23 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUNTER, Isaiah | SR | Wis.-Parkside | 23.34 | Warhawk Invitational & Multi |
| 2 | SAFO, George | FR | McKendree | 23.17 | Miner Invitational |
| 3 | SPARKS, Bryce | FR | Tennessee-Martin | 23.11 | OVC championship |
| 4 | WARD, Ja'Kobe | FR | Tennessee-Martin | 22.98 | Bellarmine Open |
| 5 | HUTCHCRAFT, Holden | FR | McKendree | 23.02 | Miner Invitational |
| 6 | JONES, Jacoby | SR | Lewis | 23.14 | |
| 7 | BARTLETT, Xzavier | JR | SE Missouri | 23.22 | Memphis Tiger Invitational |
| 8 | WARD, David | JR | Wis.-Parkside | 23.87 | Cal State LA Twilight (College/Univ) |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOHN, Omari | JR | Memphis | 22.94 | Ole Miss Classic 2023 |
| 2 | RUSHING, Cole | SO | Southern Illinois | 22.90 | Don DeNoon Invite |
| 3 | HOLLAND, Darius | JR | Tennessee-Martin | 22.89 | ASU Red Wolf Open |
| 4 | NEUMANN, Zach | SO | Wis.-Parkside | 22.85 | GLIAC Indoor Track & Field Championships |
| 5 | ALZOUBI, Sami | SO | Lewis | 22.87 | NCC Heenan Invitational |
| 6 | READNOUR, Isaac | JR | SE Missouri | 22.90 | 2023 Indiana University Relays |
| 7 | THOMPSON, Jamire | SR | Tennessee-Martin | 22.92 | OVC championship |
| 8 | ROMENS, Anthony | SR | Memphis | 22.95 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MOORE, Connor | FR | SE Missouri | 22.81 | PNC Bank Bellarmine Classic |
| 2 | SUMNER, Preston | SO | Southern Illinois | 22.68 | Don DeNoon Invite |
| 3 | BURSTON, Chase | SO | SIU Edwardsville | 22.31 | University of Charleston Golden Eagle Invitational |
| 4 | DEBOLT, Will | JR | Lewis | 22.23 | GLVC Indoor Track & Field Championships |
| 5 | VAZQUEZ, Hugo | FR | Lindenwood | 22.29 | OVC championship |
| 6 | MASSIAH, Caleb | SO | Southern Illinois | 22.66 | Illini Invitational |
| 7 | CLUVER, Maluke | Unattached | 22.80 | ||
| 8 | WILSON, Bryson | FR | Southern Illinois | 22.82 | 2023 Indiana University Relays |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PARHAM, Brenden | JR | SE Missouri | 22.04 | OVC championship |
| 2 | JOHNSON, Chris | FR | SE Missouri | 21.97 | 2023 South Alabama Invitational |
| 3 | CARROLL, Malachi | SR | McKendree | 21.56 | Lindenwood Dick Cochran Invite |
| 4 | WALKES-MILLER, Jameel | JR | Lindenwood | 21.08 | GLVC Track and Field Championships |
| 5 | NUZZO, Robert | SR | SIU Edwardsville | 21.37 | Illini Invitational |
| 6 | KOCANDA, Reese | SR | Lewis | 21.85 | Cal State LA Twilight (College/Univ) |
| 7 | THREADGILL, Lamark | FR | Southern Illinois | 22.04 | MVC Indoor Track & Field Championship |
| 8 | CHARLES, Boris | SR | Lindenwood | 22.23 | GLVC Track and Field Championships |