| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Amari | FR | DeSmet Jesuit High School | NT | Unverified: |
| 2 | WERTEL, Dylan | SR | Christian Brothers College High | 48.29 | Verified |
| 3 | BRADLEY, Hunter | JR | Vianney High School | 46.71 | Verified |
| 4 | BENNETT, Jaxon | FR | St. Louis University High School | 47.03 | Verified |
| 5 | GARRETT, John | SO | Vianney High School | 48.70 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DAILEY-JONES, De Veontay | SO | Confluence Prep Academy Charter | 45.57 | Verified |
| 2 | CAMERON, Karrell | SO | Confluence Prep Academy Charter | 43.62 | Verified |
| 3 | HURLEY, Lucas | SO | Vianney High School | 41.04 | Verified |
| 4 | SPAETE JR, Christopher | SR | Christian Brothers College High | 42.54 | Verified |
| 5 | HOUSTON, Josiah | SO | DeSmet Jesuit High School | 45.49 | Verified |
| 6 | MOORE, Matthew | SO | St. Louis University High School | 45.74 | Verified |