| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Miller Career Academy | A | Gleghorn | Morris | Nesbitt | Aaron | 46.74 | Verified |
| 2 | Vianney High School | A | Ivory | Fronabarger | Pullam | Battle | 46.11 | Verified |
| 3 | Confluence Prep Academy Charter | A | Holmes | Ward | Sherrod | Aaron | 44.59 | Verified |
| 4 | Christian Brothers College High | A | Van | Tillman | Hendrix | Cothrine | 45.00 | Unverified: |
| 5 | St. Louis University High School | A | Bailey | Baur | Bennett | Chaffee | 46.13 | Unverified: |