| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Miller Career Academy | A | Nesbitt | Walker | Aaron | Woods | NT | Verified |
| 2 | Christian Brothers College High | A | Hayden | Hendrix | Cothrine | Pauley | 1:35.77 | Unverified: Parkway North Fred Lyon Invitati |
| 3 | Confluence Prep Academy Charter | A | Lance | Ward | Holmes | Aaron | 1:33.00 | Verified |
| 4 | St. Louis University High School | A | Bailey | Baur | Jordan | Chaffee | 1:34.20 | Unverified: |
| 5 | Vianney High School | A | Pullam | Ivory | Hunsaker | Selsor | 1:38.03 | Verified |
| 6 | DeSmet Jesuit High School | A | Herring | Ramsey | Robinson | Williams | 1:45.58 | Unverified: |