| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vianney High School | A | Turmelle | Miesner | Roche | Lindsey | 4:13.54 | Verified |
| 2 | Confluence Prep Academy Charter | A | Cameron | Lance | Ward | Gambleton | 3:45.00 | Verified |
| 3 | Christian Brothers College High | A | Dubose | Hendricks | Alexander | Hayden | 3:29.00 | Unverified: |
| 4 | St. Louis University High School | A | Moore | Franks | Bognar | Mayer | 3:34.81 | Unverified: |
| 5 | DeSmet Jesuit High School | A | Houston | Terry | Ricks | Ramsey | 3:48.75 | Verified |
| 6 | Miller Career Academy | A | Aaron | Walker | Morris | Allen | NT | Unverified: |