| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NESBITT, Donnell | JR | Miller Career Academy | NT | Unverified: |
| 2 | WOODS, Devon | JR | Miller Career Academy | 26.41 | Verified |
| 3 | RICKS, Taylor | SO | DeSmet Jesuit High School | 25.44 | Verified |
| 4 | AARON, Jamar | JR | Miller Career Academy | 26.05 | Verified |
| 5 | VAN, Dylan | SR | Christian Brothers College High | NT | |
| 6 | PULLAM, Caden | FR | Vianney High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUNSAKER, Turner | JR | Vianney High School | 25.20 | Verified |
| 2 | BAUR, Jeffrey | SR | St. Louis University High School | 24.64 | Verified |
| 3 | HOLMES, James | JR | Confluence Prep Academy Charter | 24.04 | Verified |
| 4 | SELSOR, Ian | SO | Vianney High School | 24.40 | Verified |
| 5 | KING, Jason | SO | DeSmet Jesuit High School | 24.74 | Verified |
| 6 | ROBINSON, Brandon | SO | DeSmet Jesuit High School | 25.24 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BAILEY, Kameron | SR | St. Louis University High School | 23.83 | Verified |
| 2 | SIMMS, Corey | SO | Christian Brothers College High | 23.70 | Verified |
| 3 | DUBOSE, Doreon | JR | Christian Brothers College High | 23.28 | Verified |
| 4 | AARON, Khalil | JR | Confluence Prep Academy Charter | 23.32 | Verified |
| 5 | CHAFFEE, Sean | SR | St. Louis University High School | 23.74 | Verified |
| 6 | WARD, Kingston | JR | Confluence Prep Academy Charter | 24.02 | Verified |