| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Troy South MS | A | Bell | Arnold | Toedebusch | Allen | DQ | Unverified: |
| 3 | Quincy Junior High School | A | 2:18.00 | Unverified: | ||||
| 4 | Palmyra MS | A | Wisehart | Wise | Timbrook | Meyer | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Liberty Middle School (IL) | A | Parkhill | Logsdon | Ivers | Crow | NT | Unverified: |
| 3 | Kirksville Middle School | A | Summers | Treasure | Lyons | Cochran | 2:03.91 | Verified |
| 4 | Hannibal Middle School | A | Thompson | Davis | Brown | Ricker | 2:05.00 | Unverified: |
| 5 | Bowling Green Middle School | A | McMillen | Holland | Milosovich | Finley | 2:09.14 | Verified |