| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Troy South MS | A | Ilgenfritz | Stephenson | Weikel | Greco | NT | Unverified: |
| 3 | Palmyra MS | A | McNeff | Bock | Schlueter | Gibson | NT | Unverified: |
| 4 | Bowling Green Middle School | A | Bair | Hickerson | McDannold | Welch | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hannibal Middle School | A | Danner | Wilson | Dolbare | Howard | 4:28.86 | Verified |
| 2 | Kirksville Middle School | A | Reinbach | Cassady | Pierce | Ihnat | 4:12.52 | Verified |
| 3 | Troy Middle School | A | Bont | Martin | Stanek | Seymour | 4:04.08 | Verified |
| 4 | Mexico Middle School | A | Grubb | Matthews | Wilson | Bass | 4:10.86 | Verified |
| 5 | Quincy Junior High School | A | 4:24.00 | Unverified: | ||||
| 6 | Clopton Middle School | A | Myers | Palmer | Waller | Frichett | 4:49.82 | Verified |