| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Troy South MS | A | Raney | Coleman | Wright | Moore | NT | Unverified: |
| 3 | Clopton Middle School | A | Hallows | Wilson | Talbert | Beauchamp | 5:25.33 | Verified |
| 4 | Palmyra MS | A | Clark | James | Moore | Platt | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mexico Middle School | A | Hudson | Cox | Bryson | McCarty | 5:07.99 | Verified |
| 2 | Quincy Junior High School | A | 4:59.00 | Unverified: | ||||
| 3 | Troy Middle School | A | Witt | Pursifull | Bonds | Means | 4:53.76 | Unverified: |
| 4 | Liberty Middle School (IL) | A | Gilbert | McKinney | Field | Mixer | 4:54.50 | Unverified: |
| 5 | Bowling Green Middle School | A | Vanhooser | Spires | Barnes | Grote | 5:00.30 | Verified |
| 6 | Hannibal Middle School | A | Dean | Deien | Livesay | Lovercheck | 5:14.68 | Verified |