| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clopton Middle School | A | Mills | VanBebber | Weaver | Jensen | NT | Unverified: |
| 2 | Liberty Middle School (IL) | A | Parkhill | Obert | John | Ivers | 5:04.07 | Unverified: |
| 3 | Kirksville Middle School | A | Treasure | McIntosh | Davis | Danielson | 4:43.40 | Verified |
| 4 | Quincy Junior High School | A | 5:02.00 | Unverified: | ||||
| 5 | Troy South MS | A | Rust | Toedebusch | Bell | Arnold | NT | Unverified: |
| 6 | Palmyra MS | A | Pontius | Timbrook | Meyer | Reid | NT | Unverified: |