| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REEKIE, Campbell | 8 | Quincy Junior High School | 2:19.00 | Unverified: |
| 2 | STEINER, Jude | 8 | Quincy Junior High School | 2:24.00 | Unverified: |
| 3 | QUINN, Jack | 8 | Kirksville Middle School | 2:25.48 | Verified |
| 4 | MCNEFF, Ian | 8 | Palmyra MS | 2:27.57 | Verified |
| 5 | HALE, Ethan | 8 | Kirksville Middle School | 2:31.38 | Verified |
| 6 | FOWLER, Luke | 8 | Mexico Middle School | 2:33.46 | Verified |
| 7 | HICKERSON, Bennett | 8 | Bowling Green Middle School | 2:36.34 | Verified |
| 8 | LOCKE, Zach | 8 | Hannibal Middle School | 2:37.24 | Verified |
| 9 | WEIKEL, Elijah | 8 | Troy South MS | 2:37.80 | Verified |
| 10 | TRAIL, Devon | 8 | Wentzville Middle School | 2:37.89 | Verified |
| 11 | LIBER, Adrian | 8 | Bowling Green Middle School | 2:38.64 | Verified |
| 12 | MASTIN, Quinn | 8 | Hannibal Middle School | 2:42.49 | Verified |
| 13 | STEPHENSON, Gunnar | 8 | Troy South MS | 2:52.77 | Verified |
| 14 | KLAUSER, Brayden | 8 | Liberty Middle School (IL) | 2:55.13 | Unverified: |
| 15 | GONZALEZ, Alejandro | 8 | Hannibal Middle School | 2:56.03 | Verified |
| 16 | PALMER, Colmen | 8 | Clopton Middle School | 2:59.31 | Verified |
| 17 | CAMPBELL, Kessler | 8 | Hannibal Middle School | NT | Unverified: |
| 18 | TUCKER, Azariah | 8 | Hannibal Middle School | NT | Unverified: |
| 19 | HAYES, Rhett | 8 | Hannibal Middle School | NT | Unverified: |
| 20 | BORK, Austin | 8 | Hannibal Middle School | NT | Unverified: |
| 21 | DORSEY, Wyatt | 8 | Hannibal Middle School | NT | Unverified: |
| 22 | WILSON, Wyatt | 8 | Mexico Middle School | NT | Unverified: |
| 23 | BOCK, Larson | 8 | Palmyra MS | NT | Unverified: |
| 24 | GHEENS, Camden | 8 | Hannibal Middle School | NT | Unverified: |
| 25 | PENDERGRASS, Jacob | 8 | Hannibal Middle School | NT | Unverified: |
| 26 | TERRILL, Wyatt | 8 | Hannibal Middle School | NT | Unverified: |
| 27 | BOWLING-STANGLE, Calihan | 8 | Troy Middle School | NT | Unverified: |
| 28 | MASTIN, Reece | 8 | Hannibal Middle School | NT | Unverified: |