| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | POE, Eli | 7 | Quincy Junior High School | 2:24.00 | Unverified: |
| 2 | RAY, Charles | 7 | Quincy Junior High School | 2:34.00 | Unverified: |
| 3 | VINCENT, Alex | 7 | Kirksville Middle School | 2:34.79 | Verified |
| 4 | GOFF, Presley | 7 | Mexico Middle School | 2:48.01 | Verified |
| 5 | SCHUTTER, Jason | 7 | Kirksville Middle School | 3:09.29 | Verified |
| 6 | KOCH, Kade | 6 | Liberty Middle School (IL) | 3:10.40 | Unverified: |
| 7 | LORENSEN, Emmett | 7 | Palmyra MS | NT | Unverified: |
| 8 | TWEEDY, Nathan | 7 | Hannibal Middle School | NT | Unverified: |
| 9 | MURRAY, Mason | 7 | Bowling Green Middle School | NT | Unverified: |
| 10 | SCHONE, Logan | 7 | Troy Middle School | NT | Unverified: |
| 11 | PARKER, Jett | 7 | Hannibal Middle School | NT | Unverified: |
| 12 | WINTERHALTER, Otto | 7 | Troy South MS | NT | Unverified: |
| 13 | GILMORE, Luka | 7 | Hannibal Middle School | NT | Unverified: |
| 14 | WEHDE, Gracin | 6 | Troy Middle School | NT | Unverified: |
| 15 | PEZLEY, Luke | 7 | Mexico Middle School | NT | Unverified: |