| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KNUDSON, Leah | 8 | Quincy Junior High School | 2:43.00 | Unverified: |
| 2 | COX, Kaili | 8 | Mexico Middle School | 2:45.84 | Verified |
| 3 | WITT, Ryleigh | 8 | Troy Middle School | 2:46.17 | Verified |
| 4 | NEISEN, Briella | 8 | Liberty Middle School (IL) | 2:49.62 | Verified |
| 5 | MAHURIN, Crystal | 8 | Kirksville Middle School | 2:53.32 | Verified |
| 6 | MATUZ, Brenda | 8 | Mexico Middle School | 3:01.17 | Verified |
| 7 | WALTERS, Allison | 8 | Hannibal Middle School | 3:04.11 | Verified |
| 8 | MUNGER, Grace | 8 | Hannibal Middle School | 3:04.15 | Verified |
| 9 | WEBER, Adelyn | 8 | Quincy Junior High School | 3:11.00 | Unverified: |
| 10 | KURZ, Kendall | 8 | Hannibal Middle School | 3:13.64 | Verified |
| 11 | RICKARD, Hadley | 8 | Troy Middle School | 3:23.20 | Verified |
| 12 | DAVIS, Piper | 8 | Kirksville Middle School | 3:31.49 | Verified |
| 13 | HEUER, Bella | 8 | Clopton Middle School | 3:38.47 | Verified |
| 14 | CROUCH, Reagan | 8 | Troy South MS | 3:41.35 | Verified |
| 15 | WEISLOCHER, Rhiley | 8 | Troy South MS | 3:55.50 | Verified |
| 16 | SPIRES, Khloe | 8 | Bowling Green Middle School | NT | Unverified: |
| 17 | NIEMEYER, Lydia | 8 | Bowling Green Middle School | NT | Unverified: |
| 18 | CAULEY, Analise | 8 | Wentzville Middle School | NT | Unverified: |
| 19 | HALLOWS, Karsyn | 8 | Clopton Middle School | NT | Unverified: |
| 20 | HARSELL, Makayla | 8 | Hannibal Middle School | NT | Unverified: |
| 21 | PLATT, Brooklynn | 8 | Palmyra MS | NT | Unverified: |
| 22 | RAGAN, Rylie | 8 | Wentzville Middle School | NT | Unverified: |