| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KUHL, Peyton | 7 | Quincy Junior High School | 2:42.00 | Unverified: |
| 2 | DORSEY, Lyla | 7 | Hannibal Middle School | 2:47.47 | Verified |
| 3 | JOHN, Weslee | 7 | Liberty Middle School (IL) | 2:51.24 | Unverified: |
| 4 | PORTER, Tessa | 7 | Quincy Junior High School | 2:52.00 | Unverified: |
| 5 | SEEDORF, Nora | 7 | Kirksville Middle School | 2:52.42 | Verified |
| 6 | OBERT, Kenzie | 6 | Liberty Middle School (IL) | 2:56.90 | Unverified: |
| 7 | DAVIS, Shealynn | 7 | Kirksville Middle School | 3:04.34 | Verified |
| 8 | REID, Audrey | 7 | Palmyra MS | 3:12.54 | Verified |
| 9 | HADEN, Ellie | 7 | Bowling Green Middle School | 3:16.82 | Verified |
| 10 | BONVILLIAN, Ella | 7 | Hannibal Middle School | 3:27.00 | Unverified: |
| 11 | KNOCHEL, Adaline | 7 | Palmyra MS | 3:31.02 | Verified |
| 12 | VANBEBBER, Briley | 6 | Clopton Middle School | 3:35.90 | Verified |
| 13 | MCMILLEN, Lilly | 7 | Bowling Green Middle School | 3:37.32 | Verified |
| 14 | MCCARTY, Bentley | 7 | Mexico Middle School | NT | Unverified: |
| 15 | WILSON, Libby | 6 | Clopton Middle School | NT | Unverified: |
| 16 | ARNOLD, Reagan | 7 | Troy South MS | NT | Unverified: |
| 17 | MEDINA-LUCAS, Jackie | 7 | Mexico Middle School | NT | Unverified: |