| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RUST, Julia | 7 | Troy South MS | NT | Unverified: |
| 2 | RHOADS, Mackenzie | 7 | Troy Middle School | NT | Unverified: |
| 3 | MILLS, Charlotte | 6 | Clopton Middle School | NT | Unverified: |
| 4 | HOWELL, Parker | 7 | Mexico Middle School | NT | Unverified: |
| 5 | HEIMBACH, Violet | 6 | Troy Middle School | NT | Unverified: |
| 6 | MEYER, Delaina | 7 | Palmyra MS | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TOEDEBUSCH, McKenna | 7 | Troy South MS | NT | Unverified: |
| 2 | MAGRUDER, Josi | 6 | Clopton Middle School | 1:38.13 | Verified |
| 3 | BUCHANAN, Sydnee | 7 | Quincy Junior High School | 1:20.00 | Unverified: |
| 4 | LOGAN, Makenzlei | 7 | Mexico Middle School | 1:22.36 | Verified |
| 5 | LEON, Fernanda | FR | Bowling Green Middle School | 1:39.72 | Verified |
| 6 | TALLMAN, Paige | 7 | Palmyra MS | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCINTOSH, Micki | 7 | Kirksville Middle School | 1:11.93 | Verified |
| 2 | DAVIS, Adyson | 7 | Hannibal Middle School | 1:11.00 | Unverified: |
| 3 | FALCONER, Marissa | 7 | Hannibal Middle School | 1:04.00 | Verified |
| 4 | NICHOLSON, Khloe | 7 | Quincy Junior High School | 1:09.14 | Unverified: |
| 5 | DANIELSON, Eva | 7 | Kirksville Middle School | 1:11.01 | Verified |
| 6 | PARKHILL, Leila | 7 | Liberty Middle School (IL) | 1:13.66 | Unverified: |