| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Palmyra MS | A | Harper | Schlueter | Gibson | Kroeger | NT | Unverified: |
| 3 | Bowling Green Middle School | A | Doggett | Leon | Kniess | Kroeck | 56.28 | Verified |
| 4 | Clopton Middle School | A | Frichett | Waller | Rickey | Liley | 58.47 | Verified |
| 5 | Troy South MS | A | Christopherson | Toebben | Hunolt | Reynolds-Brandt | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hannibal Middle School | A | Washington | Wilson | Anderson | Dolbare | 51.93 | Verified |
| 2 | Mexico Middle School | A | Grubb | Ford | Williams | Bass | 51.42 | Verified |
| 3 | Quincy Junior High School | A | 46.40 | Unverified: | ||||
| 4 | Kirksville Middle School | A | Mbela | Cassady | Cook | Ihnat | 50.22 | Verified |
| 5 | Liberty Middle School (IL) | A | Alexander | Deege | Curry | Huber | 51.69 | Unverified: |
| 6 | Troy Middle School | A | Thompson | Wittich | Croxton | Seymour | 53.02 | Unverified: |