| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wentzville Middle School | A | Lewis | Thompson | Moore | Graham | NT | Unverified: |
| 3 | Clopton Middle School | A | Talbert | Wilson | Stanek | Caplin | 1:04.65 | Verified |
| 4 | Troy South MS | A | Coleman | Enos | Keene | Hacker | NT | Unverified: |
| 5 | Palmyra MS | A | Moore | Martin | Anderson | James | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Liberty Middle School (IL) | A | Obert | Heller | Troyer | Dyer | 59.94 | Unverified: |
| 2 | Hannibal Middle School | A | Closser | Dean | Falconer | Lovercheck | 59.65 | Verified |
| 3 | Quincy Junior High School | A | 56.68 | Unverified: | ||||
| 4 | Kirksville Middle School | A | Kiese | DeLeon | Greathouse | Kahindo | 57.10 | Verified |
| 5 | Troy Middle School | A | Benden | Hagen | Glimka | Dale | 59.80 | Unverified: |
| 6 | Bowling Green Middle School | A | Eskew | Akers | Hearn | Barnes | 1:00.20 | Verified |