| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clopton Middle School | A | Wilson | Mills | Magruder | Mills | NT | Unverified: |
| 3 | Palmyra MS | A | Wise | Tallman | Hoerr | Chapman | NT | Unverified: |
| 4 | Troy Middle School | A | Reynolds | Rhoads | Yang | Heimbach | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Liberty Middle School (IL) | A | Geise | Shaffer | Curry | Hackamack | 1:02.90 | Unverified: |
| 2 | Hannibal Middle School | A | Thompson | Davis | Brown | Ricker | 59.65 | Verified |
| 3 | Kirksville Middle School | A | Summers | Lyons | Jordan | Moore | 57.10 | Verified |
| 4 | Quincy Junior High School | A | 58.52 | Unverified: | ||||
| 5 | Bowling Green Middle School | A | Finley | Milosovich | Lovell | McMillen | 1:00.20 | Verified |