| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | COOK, Skyler | 8 | Kirksville Middle School | 5:16.01 | Verified |
| 2 | STEINER, Jude | 8 | Quincy Junior High School | 5:19.00 | Unverified: |
| 3 | MONDON, Austin | 8 | Quincy Junior High School | 5:25.00 | Unverified: |
| 4 | MASTIN, Quinn | 8 | Hannibal Middle School | 5:34.93 | Verified |
| 5 | LOCKE, Zach | 8 | Hannibal Middle School | 5:39.94 | Verified |
| 6 | HICKERSON, Bennett | 8 | Bowling Green Middle School | 5:41.74 | Verified |
| 7 | PIERCE, Colton | 8 | Kirksville Middle School | 5:48.20 | Verified |
| 8 | JOHNSON, Montrez | 8 | Mexico Middle School | 5:50.21 | Verified |
| 9 | TRAIL, Devon | 8 | Wentzville Middle School | 5:59.53 | Verified |
| 10 | WILSON, Wyatt | 8 | Mexico Middle School | 6:06.95 | Verified |
| 11 | LYNN, Aiden | 8 | Palmyra MS | 6:30.41 | Verified |
| 12 | LIBER, Adrian | 8 | Bowling Green Middle School | 6:31.64 | Verified |
| 13 | PALMER, Colmen | 8 | Clopton Middle School | 6:32.11 | Verified |
| 14 | GRECO, Drew | 8 | Troy South MS | 6:56.08 | Verified |
| 15 | GONZALEZ, Alejandro | 8 | Hannibal Middle School | 6:59.40 | Verified |
| 16 | TERRILL, Wyatt | 8 | Hannibal Middle School | 7:15.59 | Verified |
| 17 | CAMPBELL, Kessler | 8 | Hannibal Middle School | 8:37.73 | Verified |
| 18 | MCCASKEY, Coby | 8 | Palmyra MS | NT | Unverified: |
| 19 | PENDERGRASS, Jacob | 8 | Hannibal Middle School | NT | Unverified: |
| 20 | HAYES, Rhett | 8 | Hannibal Middle School | NT | Unverified: |
| 21 | BORK, Austin | 8 | Hannibal Middle School | NT | Unverified: |
| 22 | GHEENS, Camden | 8 | Hannibal Middle School | NT | Unverified: |
| 23 | ILGENFRITZ, Mel | 8 | Troy South MS | NT | Unverified: |
| 24 | HAYDEN, Jackson | 8 | Clopton Middle School | NT | Unverified: |
| 25 | TUCKER, Azariah | 8 | Hannibal Middle School | NT | Unverified: |
| 26 | MASTIN, Reece | 8 | Hannibal Middle School | NT | Unverified: |