| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | POE, Eli | 7 | Quincy Junior High School | 5:10.00 | Unverified: |
| 2 | RAY, Charles | 7 | Quincy Junior High School | 5:20.00 | Unverified: |
| 3 | VINCENT, Alex | 7 | Kirksville Middle School | 5:56.92 | Verified |
| 4 | LORENSEN, Emmett | 7 | Palmyra MS | 6:00.02 | Verified |
| 5 | LINDSAY, Colin | 6 | Clopton Middle School | 6:07.69 | Verified |
| 6 | LIVINGSTON, Oliver | 7 | Troy Middle School | 6:08.59 | Unverified: |
| 7 | BARNES, Truitt | 7 | Palmyra MS | 6:14.96 | Verified |
| 8 | DEBLAUW, Noah | 7 | Kirksville Middle School | 6:45.48 | Verified |
| 9 | KOCH, Kade | 6 | Liberty Middle School (IL) | 6:45.70 | Unverified: |
| 10 | PARKER, Jett | 7 | Hannibal Middle School | NT | Unverified: |
| 11 | MURRAY, Mason | 7 | Bowling Green Middle School | NT | Unverified: |
| 12 | HERNANDEZ-JARQUIN, Israel | 7 | Mexico Middle School | NT | Unverified: |
| 13 | SCHONE, Logan | 7 | Troy Middle School | NT | Unverified: |
| 14 | BURETTA, Kaden | 7 | Troy South MS | NT | Unverified: |
| 15 | TWEEDY, Nathan | 7 | Hannibal Middle School | NT | Unverified: |