| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KNUDSON, Leah | 8 | Quincy Junior High School | 6:09.00 | Unverified: |
| 2 | NEISEN, Briella | 8 | Liberty Middle School (IL) | 6:16.00 | Unverified: |
| 3 | BUNTE, Laina | 8 | Quincy Junior High School | 6:23.06 | Verified |
| 4 | MUNGER, Grace | 8 | Hannibal Middle School | 6:31.29 | Verified |
| 5 | KURZ, Kendall | 8 | Hannibal Middle School | 6:31.60 | Verified |
| 6 | MATUZ, Brenda | 8 | Mexico Middle School | 6:46.43 | Verified |
| 7 | MAHURIN, Crystal | 8 | Kirksville Middle School | 6:58.43 | Verified |
| 8 | ENLOE, Macy | 8 | Clopton Middle School | 6:59.21 | Unverified: |
| 9 | RICKARD, Hadley | 8 | Troy Middle School | 7:15.42 | Verified |
| 10 | WALTERS, Allison | 8 | Hannibal Middle School | 7:24.44 | Verified |
| 11 | DAVIS, Piper | 8 | Kirksville Middle School | 7:37.71 | Verified |
| 12 | WEBB, Jenna | 8 | Palmyra MS | NT | Unverified: |
| 13 | HARSELL, Makayla | 8 | Hannibal Middle School | NT | Unverified: |
| 14 | RAGAN, Rylie | 8 | Wentzville Middle School | NT | Unverified: |