| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KUHL, Peyton | 7 | Quincy Junior High School | 5:51.00 | Unverified: |
| 2 | JOHN, Weslee | 7 | Liberty Middle School (IL) | 6:01.49 | Unverified: |
| 3 | DORSEY, Lyla | 7 | Hannibal Middle School | 6:03.66 | Verified |
| 4 | SEEDORF, Nora | 7 | Kirksville Middle School | 6:08.60 | Verified |
| 5 | MACDONOUGH, Abigail | 7 | Quincy Junior High School | 6:14.00 | Unverified: |
| 6 | OBERT, Kenzie | 6 | Liberty Middle School (IL) | 6:18.96 | Unverified: |
| 7 | KOTTWITZ, Amber | 7 | Kirksville Middle School | 6:42.39 | Verified |
| 8 | VANHOOSER, McKinley | 7 | Bowling Green Middle School | 6:57.09 | Verified |
| 9 | PONTIUS, Leah | 7 | Palmyra MS | 7:00.00 | Unverified: |
| 10 | REID, Audrey | 7 | Palmyra MS | 8:00.00 | Unverified: |
| 11 | BONVILLIAN, Ella | 7 | Hannibal Middle School | NT | Unverified: |
| 12 | MEDINA-LUCAS, Jackie | 7 | Mexico Middle School | NT | Unverified: |
| 13 | JENSEN, Bailee | 6 | Clopton Middle School | NT | Unverified: |
| 14 | MILLS, Savannah | 6 | Clopton Middle School | NT | Unverified: |