| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Troy South MS | A | Enos | Coleman | Moore | Wilson | NT | Unverified: |
| 3 | Wentzville Middle School | A | Graham | McGrail | Jackson-Thomas | Lewis | NT | Unverified: |
| 4 | Hannibal Middle School | A | Closser | Dean | Falconer | Livesay | NT | Unverified: |
| 5 | Palmyra MS | A | Hudson | Hamlin | Plunkett | James | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clopton Middle School | A | Talbert | Wilson | Stanek | Caplin | 2:12.30 | Verified |
| 2 | Quincy Junior High School | A | 2:05.00 | Unverified: | ||||
| 3 | Liberty Middle School (IL) | A | Jensen | Mixer | Neisen | John | 2:00.60 | Unverified: |
| 4 | Kirksville Middle School | A | DeLeon | Greathouse | DeLeeuw | Kahindo | 2:03.91 | Verified |
| 5 | Bowling Green Middle School | A | Eskew | Barnes | Akers | Spires | 2:09.14 | Verified |
| 6 | Troy Middle School | A | Eason | Hagen | Martin | Pursifull | NT | Unverified: |