| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Riverview Gardens High School | B | NT | Unverified: | ||||
| 3 | Hazelwood West High School | B | NT | Unverified: | ||||
| 4 | Hazelwood East High School | A | Burks | III | Johnson | Kirby | NT | Unverified: |
| 5 | Hazelwood East High School | B | Anderson | Cole | Jarrett | Little | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | STEAM Academy at McCluer South-B | B | Johnson | Boyd | Grimes | Smith | 1:36.99 | Unverified: |
| 2 | Hazelwood Central High School | A | Watson | Funches | Trotter | Donald | 1:31.97 | Verified |
| 3 | Riverview Gardens High School | A | 1:29.00 | Unverified: | ||||
| 4 | Hazelwood Central High School | B | Hunter | Reed | Mitchell | Bright | 1:31.97 | Verified |
| 5 | STEAM Academy at McCluer South-B | A | Moreland-Bell | Coleman | Ivy | Evans | 1:32.66 | Unverified: |
| 6 | Hazelwood West High School | A | NT | Unverified: |