| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood West High School | A | NT | Unverified: | ||||
| 2 | Hazelwood Central High School | A | Hunter | Atkins | Funches | Williams | 3:49.44 | Verified |
| 3 | STEAM Academy at McCluer South-B | A | Moreland-Bell | Coleman | Hines | Ivy | 3:36.81 | Unverified: |
| 4 | Hazelwood East High School | A | III | Johnson | Sledge | Watson | 3:38.53 | Verified |
| 5 | Riverview Gardens High School | A | NT | Unverified: |