| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCCLELLAND, Amick | SO | Quincy (Sr.) | 5:41.68 | Verified |
| 2 | PUTERBAUGH, Jordan | FR | Hannibal High School | 6:11.86 | Verified |
| 3 | RAMSEY, Maggie | FR | Mexico High School | 6:12.00 | Verified |
| 4 | BIBB, Dianna | SO | Bowling Green High School | 6:32.51 | Verified |
| 5 | BERHORST, Piper | FR | Hannibal High School | 6:34.00 | Verified |
| 6 | WIGFALL, Riah | SO | Hannibal High School | 6:52.43 | Verified |
| 7 | BATES, Emilia | SO | Hannibal High School | 7:05.60 | Verified |
| 8 | PLENGE, Irelyn | FR | Clark County High School | 7:17.72 | Verified |
| 9 | LINDGREN, Elise | FR | Quincy (Sr.) | 7:29.55 | Verified |
| 10 | BONVILLIAN, Ava | SO | Hannibal High School | NT |