| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Quincy (Sr.) | A | Bruening | Alexandre | Torres | Robinson | 1:48.88 | Unverified: |
| 2 | Bowling Green High School | A | Lewis | Grote | Ream | Niemeyer | 1:39.00 | Verified |
| 3 | Hannibal High School | A | Odom | Worthington | 1:32.75 | Verified | ||
| 4 | Mexico High School | A | Rowan | Potter | Grimes | Charlie) Fisher | 1:37.96 | Verified |
| 5 | Clark County High School | A | Randle | Rodgers | PARKER | BURGESS | 1:39.53 | Verified |