| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SEIBERT, Emmaleigh | FR | Mexico High School | NT | Unverified: |
| 3 | CHANDLER, Haiden | FR | Bowling Green High School | 22.96 | Verified |
| 4 | NGUYEN, Hannah | FR | Hannibal High School | NT | Unverified: |
| 5 | THOMAS, Alizia | FR | Hannibal High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CRIDER, Araeyah | SO | Quincy (Sr.) | 21.84 | Verified |
| 2 | CHARLTON, Sydney | SO | Bowling Green High School | 21.40 | Verified |
| 3 | BROWN, Riyenn | SO | Hannibal High School | 17.57 | Verified |
| 4 | MCDOWELL, Ericka | FR | Clark County High School | 20.51 | Verified |
| 5 | ALEXANDER, Ja'Nylah | SO | Quincy (Sr.) | 21.64 | Verified |
| 6 | RILEY, Chloe | FR | Hannibal High School | 22.18 | Verified |