| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Bowling Green High School | A | Bibb | Korte | Turner | Dawson | 5:03.11 | Verified |
| 3 | Quincy (Sr.) | A | Alexander | McClelland | Summers | Venvertloh | 4:20.29 | Verified |
| 4 | Hannibal High School | A | Ball | Groth | Karr | Coniglio | 4:26.09 | Verified |