| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MACDONOUGH, Cameron | SO | Quincy (Sr.) | 2:06.43 | Verified |
| 2 | GRIMES, Tyler | SO | Mexico High School | 2:06.77 | Verified |
| 3 | THOMPSON, Jonas | FR | Hannibal High School | 2:10.75 | Verified |
| 4 | STEINKAMP, Lane | FR | Quincy (Sr.) | 2:17.62 | Verified |
| 5 | MATLOCK, Daniel | FR | Bowling Green High School | 2:19.65 | Verified |
| 6 | LOCKE, Jared | SO | Hannibal High School | 2:26.54 | Verified |
| 7 | SCHUETZ, Gavin | SO | Clark County High School | 2:28.41 | Verified |
| 8 | MARTIN, Ismael | SO | Hannibal High School | 2:39.71 | Verified |
| 9 | HIGGINS, Landon | SO | Mexico High School | 2:45.04 | Verified |
| 10 | KING, Axell | FR | Clark County High School | 2:50.57 | Verified |